Journal: bioRxiv

Article Title: De novo protein design enables precise induction of functional antibodies in vivo

doi: 10.1101/685867

Figure Lengend Snippet: A) Multiple sequence alignment of S4_2 sequences that were enriched after yeast screening for protease resistance and binding to 101F. Sequences are colored according to a BLOSUM62 score matrix. B) Circular dichroism spectra for 12 designs of the S4_2 design series that were enriched for protease resistance and binding to 101F in the yeast display selection assay. Black: spectrum at 20 °C. Red: spectrum at 90 °C. C) SPR sensorgrams for binding to 101F for the same designs. 101F IgG was immobilized on the CM5 sensor chip surface, and the designs were flown as analyte.

Article Snippet: Ligands were immobilized on a CM5 chip (GE Healthcare # 29104988) via amine coupling.

Techniques: Sequencing, Binding Assay, Selection